Nanosecond time-resolved emission spectroscopy of a fluorescence probe adsorbed to L-alpha-egg lecithin vesicles

Time-resolved fluorescence spectroscopy

Time Resolved Spectroscopy of a Nanosecond Pulsed Discharge in Water

A time revolved spectroscopic study of the emission phases occurring throughout a nanosecond pulsed discharge in water is presented. Discharged are ignited in deionized water via a submerged pin-t-plane electrode configuration by application of 24kV pulses with a rise time of 4ns and a duration of 10ns. Optical emission spectroscopy of the plasma radiation is performed, revealing a very complex...

Osmotic shrinkage of giant egg-lecithin vesicles.

Osmotic shrinkage of giant egg-lecithin vesicles was observed by phase-contrast microscopy. The vesicles remained or became spherical when shrinking. Small and thick-walled vesicles formed visible fingers attached to the sphere. The water permeability of the single bilayer was found to be 41 micrometers/s. A variety of observations indicate that osmosis induces a parallel lipid flow between the...

Time-Resolved Fluorescence Spectroscopy with LabView

1.1 Fluorescence phenomenon The absorption and subsequent emission of light by organic and inorganic specimens is typically the result of physical phenomena known as luminescence, which occurs at electronically excited states. Luminescence is formally divided into two categories: fluorescence and phosphorescence, depending on the nature of the excited state. Fluorescence occurs when a photon ex...

Femtosecond time-resolved optical pump-probe spectroscopy at kilohertz-scan-rates over nanosecond-time-delays without mechanical delay line

We demonstrate a technique for femtosecond time-resolved optical pump-probe spectroscopy that allows to scan over a nanosecond time delay at a kilohertz scan rate without mechanical delay line. Two mode-locked femtosecond lasers with approximately 1 GHz repetition rate are linked at a fixed difference frequency of fR=11 kHz. One laser delivers the pump pulses, the other provides the probe pulse...